NCI-H1299-Luc cell line

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location: Home > Products > Luciferase Stable Cell Lines > NCI-H1299-Luc
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  • NCI-H1299-Luc NCI-H1299-Luc-NCI-H1299-Luc
  • NCI-H1299-Luc cell line NCI-H1299-Luc-产品图
NCI-H1299-Luc Copy Product Info
Catalog#: YC-D010-Luc-P
Size: 1*10^6
Culture method: 88%RPMI-1640+10% FBS+1%Glutamax+1%Sodium Pyruvate
  • NCI-H1299-Luc NCI-H1299-Luc-NCI-H1299-Luc
  • NCI-H1299-Luc cell line NCI-H1299-Luc-产品图
NCI-H1299-Luc Copy Product Info
Catalog#: YC-D010-Luc-P
Size: 1*10^6
Culture method: 88%RPMI-1640+10% FBS+1%Glutamax+1%Sodium Pyruvate

Detailed Product Information

Cell Info

Product category Luciferase Stable Cell Lines Mycoplasma test negative
Cell Morphology Epithelial-like, adherent Culture method 88%RPMI-1640+10% FBS+1%Glutamax+1%Sodium Pyruvate
Passage ratio 1:2~1:3 Species Human
Reporter gene Luciferase Resistance gene puro
Accession
CVCL_C8W0(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Product category
Luciferase Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Epithelial-like, adherent
Culture method
88%RPMI-1640+10% FBS+1%Glutamax+1%Sodium Pyruvate
Passage ratio
1:2~1:3
Species
Human
Reporter gene
Luciferase
Resistance gene
puro
Accession
CVCL_C8W0(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.

Description

Luciferase is the general name of a class of enzymes that catalyze the oxidation and luminescence of luciferin or fatty aldehydes in organisms. Currently, firefly luciferase is the most commonly used enzyme in scientific research. This enzyme can catalyze the oxidative decarboxylation of D2 luciferin, produce activated oxidized luciferin, and emit reddish biological fluorescence. Because luciferase is based on the mutual reaction between enzyme and substrate, it has the characteristics of strong specificity, high sensitivity and signal-to-noise ratio, as well as strong penetration ability of biological fluorescent tissue. Ubigene's NCI-H1299-Luc cell line was constructed on the basis of wild-type NCI-H1299 cells by lentiviral transduction, which can stably express firefly luciferase for a long time. This cell line has low passage, high activity and good condition, and can be directly used for in vivo cell injection, which can well help you with the research of transcription factor regulation mechanism, in vivo imaging, cell tracking and other experiments.
luc产品描述
Imaging process of stable tumor cell lines in vivo
Luciferase validation
Sample Compound
pore 1
Compound
pore 2
Compound
pore 3
Average Expressed
multiple
NCI-H1299-Luc 187146 170651 185028 180941.667 333.636
NCI-H1299 489 589 549 542.333
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:NCI-H1299
STR Info (Cell bank)
Cell Line:NCI-H1299-Luc2-tdT-2
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 12121212
D7S820 10101010
D16S539 12131213
VWA 16181618
TH01 69.369.3
AMEL XXXX
TPOX 8888
CSF1PO 12121212
D12S391 2122
FGA 2020
D2S1338 2324
D21S11 32.232.2
D18S51 1616
D8S1179 1013
D3S1358 1717
D6S1043 1111
PENTAE 1111
D19S433 1414
PENTAD 1313
D1S1656 1215
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • icon
    Easy to use
    Luciferase Stable Cell Line in our cell bank can stably express luciferase. It can be directly used for in vivo injection to detect the tumorigenicity of cells.
  • icon
    Sensitive accurate
    Luciferase Stable Cell Line in our cell bank have high specificity and high sensitivity, enables high imaging quality, and its luminescence intensity can be accurately quantified.
  • icon
    Curated cells
    Our Luciferase Stable Cell Lines have been optimized and have low passages, high activity and good cell condition, applicable for detection or tumorigenesis experiment.

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